Tech Chat

Customer:
I am looking at the withdrawal device, part #LW8021 for a Lab 50 dewar.

Would this fit on an Elan 2 millineum 2000 dewar that is attached to the Elan 2 nitrogen generator. Our goal is to transfer liquid nitrogen that is made, from the millineum 2000 dewar into a Lab 30 dewar.  Would this withdrawal device fit and would it allow for that volume of transfer?

Custom BioGenic Systems:
For the withdrawal device to work it must be tightened down to seal and build pressure for transfer.  I imagine anything else attached to the dewar would break that seal.

Customer:
Thank you.  Do you have any suggestions on how we can successfully and safely transfer the liquid nitrogen that is made from the large dewar attached to the machine to a smaller dewar that we keep downstairs in the medical assistants station? Once the liquid nitrogen is processed by the Elan 2 and fills the dewar that is attached, we can move that dewar.  It can be taken off of the stand in order to dump into another container/dewar.  However, it is extremely heavy and cumbersome and we would like to find some kind of withdrawal device to attach to the top of that dewar that would allow transfer of the nitrogen into a smaller container.  Any suggestions?

Custom BioGenic Systems:
You need pressure to transfer LN2 and you lose LN2 every time it is transferred to what is called pressure drop.  Really having two dewars that you could just rotate out full/empty would be easiest.  A withdrawal device would work if the dewar was removed from the generator.  Then the withdrawal device could be installed and it would require perhaps an hour to build pressure enough for transfer.

Customer:
Thank you.  That is what I needed to know.  I appreciate your help.

_________

Customer:
Good morning,

I am interested in purchasing a few probes for our control rate freezer.  A little help?
Custom BioGenic Systems:
Sure, for vials or bags?

Customer:
Bags

Custom BioGenic Systems:
Part # 42010.   Description: Probe, TP10 Series L Ribbon for Cryo-Bags.

Customer:
Great.  So if you know what it looks like can you confirm that the sample temp reading end is square?

Custom BioGenic Systems:
Yes, it is square, orange in color.

Customer:
Perfect. Thank you, have a good day.

Custom BioGenic Systems:
You do the same.

_________

Customer:
Dispenser for liquid nitrogen is not holding pressure.  There is still about 8 inches of liquid nitrogen in the tank.  Any thoughts on what the problem might be?
Custom BioGenic Systems:
When was it last filled?

Customer:
2 weeks ago

Custom BioGenic Systems:
When was it last working?

Customer:
Yesterday

Custom BioGenic Systems:
Is the pressure relief petcock valve closed?

Customer:
How does one know it’s closed?

Custom BioGenic Systems:
Turn it all the way clockwise, it stops spinning.  The “wing nut” looking brass thing.

Customer:
Thanks I was just going to ask.

Customer:
They got it to work.

Custom BioGenic Systems:
Did anyone tighten it (ratcheting handle on top) more after it was last used?   Most common problem is usually over tightening.  It causes the cork to bulge and not seal to build pressure.

________

Customer:
How long will it take to fill a S1500 freezer for the first time?
Custom BioGenic Systems:
About an hour provided the pressure is 22 psi.

Customer:
OK and how much LN2 will it take? I have a 160, just want to make sure that is enough for first time.

Custom BioGenic Systems:
The initial fill always uses the most LN2 so I would plan to have a 2nd nearby.

Customer:
OK, didn’t know that, will it hold the 160 I put in it if I don’t get the other till later?

Custom BioGenic Systems:
How long before you get another supply tank?
What level are you filling to?
Your use rate is higher for the first three days while attempting to cool the cylinder.

Customer:
A day, or maybe even later today.

Custom BioGenic Systems:
What level are you filling to?

Customer:
6 inches?

Custom BioGenic Systems:
You should be fine, it could even last a few days but you for sure want a new supply on Friday by the end of the day.

Customer:
Cool, thanks so much.

Custom BioGenic Systems:
What would be your low level?  4″?

Customer:
Yes, low is 4.

Custom BioGenic Systems:
If you get a new supply by the next 2-3 days you will be fine.  Just be sure to perform the first fill with the lid open to avoid any excessive thermal stresses from damaging the lid.  After the initial fill you can leave it closed indefinitely.

Customer:

Thank you so much, have a great day.

Custom BioGenic Systems:
Thank you.  Please let us know if you have any further questions.

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